The effect of metformin on neutrophil recruitment in carrageenan-induced peritonitis in mice

Abstract

Objective: An acute inflammation is immediate and rudimental responses to a destructive factor and neutrophils have a basic role in immunoinflammatory responses. It has been shown that AMPK (5-Amp Activated protein Kinase) exists in neutrophils and its activation reduces the activity of neutrophils and can be a suitable pharmacological target in curing the acute inflammatory procedures. We aimed to study the effect of metformin a pharmacological activator of AMPK on leukocytes accumulation and neutrophil activity in peritonits induced by carrageenan in mice. Methods: Male Albino mice (25-30g) received orally 25,50 and 100 mg/kg metformin one hour prior to induction of peritonites by intraperitoneal injection of carrageenan 1% (0.25ml). Control animal recieved saline four hours after peritonites induction. Leukocytes were counted and neutrophils differentiation and myeloperoxidase activity were determined in the exudate. Results: The results of this study demonstrated that acute treatment of mice with single dose (50mg/kg) of Metformin significantly reduced leukocytes accumulation and neutrophil activity. Conclusion: Metformin noticabaly reduces the acute activation of neutrophiles of Ampk pathwaye by metformin is considered as a possible mechanism for this anit-inflammatory effect.