نمایش پرونده ساده آیتم

dc.contributor.authorSoleymani, J
dc.contributor.authorHasanzadeh, M
dc.contributor.authorSomi, MH
dc.contributor.authorOzkan, SA
dc.contributor.authorJouyban, A
dc.date.accessioned2018-08-26T09:37:36Z
dc.date.available2018-08-26T09:37:36Z
dc.date.issued2018
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/58123
dc.description.abstractIn recent years, study of folate receptor (FR) expression related to targeting, drug delivery and counting of tumoral cells have been followed. In this work, a fast and simple strategy was reported to determine the FR expressed cancer cells based on the selective bonding of the folic acid/folate (FA) to the FR-positive tumor cells. The folate decorated Nitrogen-doped graphene quantum dots (N-GQDs) were utilized as selective targeting of the MKN 45 cells. Fluorescent microscopy imaging investigations revealed that the produced FA conjugated N-GQDs could specifically attach to the target FR-positive tumor cells. Due to the fluorescence emission of N-GQDs, the developed cytosensor is free from attaching any fluorescent ligand i.e. Rhodamine B to capture the florescence microscopy images and also flow cytometry analysis. The fabricated cytosensor possesses a dynamic range from 100 to 7.0 -- 104 cell·mL?1 with high selectivity. Furthermore, the cytosensor also could visualized the MCF 7 and HT 29 cells where the dynamic ranges were 100 to 1.0 -- 104 and 500 to 4.0 -- 104 cells·mL?1, respectively. In vitro toxicity tests has shown low toxicity of the synthesized N-GQDs where the minimum viability is 68%. The proposed FA-N-GQDs based cytosensor provides a novel platform for detection of MKN 45, HT 29 and MCF 7 cancer cell lines which could be used in multi-channel cancer diagnosis biodevice. © 2018
dc.language.isoEnglish
dc.relation.ispartofInternational Journal of Biological Macromolecules
dc.subjectfolate receptor
dc.subjectfolic acid
dc.subjectgraphene
dc.subjectnitrogen
dc.subjectquantum dot
dc.subjectrhodamine B
dc.subjectArticle
dc.subjectcancer cell
dc.subjectcancer diagnosis
dc.subjectcell targeting
dc.subjectcell viability
dc.subjectcontrolled study
dc.subjectcytosensing
dc.subjectHT-29 cell line
dc.subjectin vitro study
dc.subjectMCF-7 cell line
dc.subjectMKN45 cell line
dc.subjectprocedures concerning cells
dc.subjectprotein expression
dc.titleTargeting and sensing of some cancer cells using folate bioreceptor functionalized nitrogen-doped graphene quantum dots
dc.typeArticle
dc.citation.volume118
dc.citation.spage1021
dc.citation.epage1034
dc.citation.indexScopus
dc.identifier.DOIhttps://doi.org/10.1016/j.ijbiomac.2018.06.183


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