Misregulaton of the dependence receptor DCC and its upstream lincRNA, LOC100287225, in colorectal cancer

Abstract

Background: Long non-coding RNAs (lncRNAs), a class of regulatory RNAs, play a major role in various cellular processes. Long intergenic non-coding RNAs (lincRNAs), a subclass of lncRNAs, are involved in the trans- and cis-regulaton of gene expression. In the case of cis-regulaton, by recruitng chromatn-modifying complexes, lincRNAs influence adjacent gene expression. Methods: We used quanttatve real-tme reverse-transcripton polymerase chain reacton (qRT-PCR) to evaluate the coexpression of LOC100287225, a lincRNA, and DCC, one of its adjacent genes that is often decreased in colorectal cancer, in pairs of tumor and adjacent tumor-free tssues of 30 colorectal cancer patents. Results: The qRT-PCR results revealed the misregulaton of these genes during tumorigenesis. Their relatve expression levels were significantly lower in tumor tssues than adjacent tumor-free tssues. However, the analysis found no significant correlaton between reduced expression of these genes. Conclusions: Our study demonstrated the concurrent misregulaton of DCC and LOC100287225 in colorectal cancer. أ¯?آ½ 2015 Wichtg Publishing.