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dc.contributor.authorRoushangar Zineh, B
dc.contributor.authorShabgard, MR
dc.contributor.authorRoshangar, L
dc.date.accessioned2018-08-26T09:01:08Z
dc.date.available2018-08-26T09:01:08Z
dc.date.issued2018
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/54977
dc.description.abstractUse of artificial cartilage due to its poor regenerative characteristics is a challenging issue in the field of tissue engineering. In this regard, three-dimensional printing (3D) technique because of its perfect structural control is one of the best methods for producing biological scaffolds. Proper biomaterials for cartilage repairs with good mechanical and biological properties and the high ability for 3D printing are limited. In this paper, a novel biomaterial consisting of Alginate (AL), Methylcellulose (MC), Halloysite Nanotube (HNT), and Polyvinylidene Fluoride (PVDF) was printed and characterized for cartilage scaffold applications. Calcium chloride (CaCl2) was used as a crosslinker for biomaterial after printing. Scanning Electron Microscopy (SEM), Energy-Dispersive X-Ray Spectroscopy (EDX), X-Ray Diffraction (XRD), Fourier-Transform Infrared Spectroscopy (FT-IR), Differential Scanning Calorimetry (DSC), tensile and compressive tests, chondrocytes seeding, cells staining, and MTT assay were carried out in the present work. The results show that in constant concentrations of AL, MC, and PVDF (40 mg/ml AL, 30 mg/ml MC, and 1% PVDF) when concentration of HNT increased from 20 mg/ml (S2) to 40 mg/ml (S14) tensile strength increased from 164 up to 381 kPa and compressive stress increased from 426 up to 648 kPa. According to spectroscopy and calorimetry results, Biomaterial shows an amorphous structure with good miscibility and a high percentage of water in its structure. PVDF reduces mechanical properties by 7% while increases cell viability by 8.75%. Histological studies and MTT assay results showed a high improvement in the percentage of living cells at the first 4 days of cell cultivation. é 2018 Elsevier B.V.
dc.language.isoEnglish
dc.relation.ispartofMaterials Science and Engineering C
dc.subject3D printers
dc.subjectAlginate
dc.subjectBiomaterials
dc.subjectBiomechanics
dc.subjectCalcium chloride
dc.subjectCalorimeters
dc.subjectCartilage
dc.subjectCells
dc.subjectCytology
dc.subjectDifferential scanning calorimetry
dc.subjectEnergy dispersive spectroscopy
dc.subjectFluorine compounds
dc.subjectFourier transform infrared spectroscopy
dc.subjectKaolinite
dc.subjectNanotubes
dc.subjectScaffolds
dc.subjectScanning electron microscopy
dc.subjectStructural dynamics
dc.subjectTensile strength
dc.subjectYarn
dc.subjectArtificial cartilages
dc.subjectBiological performance
dc.subjectBiological properties
dc.subjectEnergy dispersive X ray spectroscopy
dc.subjectFourier transform infra red (FTIR) spectroscopy
dc.subjectHalloysite nanotubes
dc.subjectMethylcellulose
dc.subjectPolyvinylidene fluorides
dc.subjectScaffolds (biology)
dc.titleMechanical and biological performance of printed alginate/methylcellulose/halloysite nanotube/polyvinylidene fluoride bio-scaffolds
dc.typeArticle
dc.citation.volume92
dc.citation.spage779
dc.citation.epage789
dc.citation.indexScopus
dc.identifier.DOIhttps://doi.org/10.1016/j.msec.2018.07.035


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