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Leuconostoc mesenteroides-derived anticancer pharmaceuticals hinder inflammation and cell survival in colon cancer cells by modulating NF-?B/AKT/PTEN/MAPK pathways

dc.contributor.authorZununi Vahed, S
dc.contributor.authorBarzegari, A
dc.contributor.authorRahbar Saadat, Y
dc.contributor.authorGoreyshi, A
dc.contributor.authorOmidi, Y
dc.date.accessioned2018-08-26T08:59:59Z
dc.date.available2018-08-26T08:59:59Z
dc.date.issued2017
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/54865
dc.description.abstractPromising results from different studies on the effect of probiotics in cancer prevention and therapy have so far been reported. However, the molecular mechanism of the interaction of probiotics with cancer cells is yet to be fully understood. In the present study, Leuconostoc mesenteroides was isolated from traditional dairy products, and its probiotic characteristics were determined. HT-29 cells were treated with conditioned-medium of designated bacteria and the cell apoptosis was studied at cellular and molecular level using DAPI staining, flow cytometry, DNA ladder assays, and real-time quantitative-PCR (q-PCR). Based on our findings, L. mesenteroides promoted apoptosis in colon cancer cell line by upregulation of MAPK1, Bax, and caspase 3, and downregulation of AKT, NF-?B, Bcl-XL expressions and some key oncomicroRNAs such as miRNA-21 and miRNA-200b significantly (p ? 0.03). The results indicated the likelihood of the examined probiotic as an alternative or complementary treatment modality in signaling-targeted cancer therapy. é 2017
dc.language.isoEnglish
dc.relation.ispartofBiomedicine and Pharmacotherapy
dc.subjectcaspase 3
dc.subjectimmunoglobulin enhancer binding protein
dc.subjectmicroRNA 200b
dc.subjectmicroRNA 21
dc.subjectmitogen activated protein kinase
dc.subjectmitogen activated protein kinase 1
dc.subjectphosphatidylinositol 3,4,5 trisphosphate 3 phosphatase
dc.subjectprobiotic agent
dc.subjectprotein Bax
dc.subjectprotein bcl xl
dc.subjectprotein kinase B
dc.subjectantineoplastic agent
dc.subjectapoptosis regulatory protein
dc.subjectcaspase 3
dc.subjectimmunoglobulin enhancer binding protein
dc.subjectmicroRNA
dc.subjectmitogen activated protein kinase
dc.subjectphosphatidylinositol 3,4,5 trisphosphate 3 phosphatase
dc.subjectprotein kinase B
dc.subjectPTEN protein, human
dc.subjectantiinflammatory activity
dc.subjectapoptosis
dc.subjectArticle
dc.subjectcancer cell
dc.subjectcancer inhibition
dc.subjectcell survival
dc.subjectcolon cancer
dc.subjectconditioned medium
dc.subjectcontrolled study
dc.subjectdown regulation
dc.subjectflow cytometry
dc.subjectHT-29 cell line
dc.subjectLeuconostoc mesenteroides
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectprotein expression
dc.subjectreal time polymerase chain reaction
dc.subjectupregulation
dc.subjectcell survival
dc.subjectcolon tumor
dc.subjectdrug effect
dc.subjecthuman
dc.subjectinflammation
dc.subjectLeuconostoc mesenteroides
dc.subjectmetabolism
dc.subjectsignal transduction
dc.subjecttumor cell line
dc.subjectAntineoplastic Agents
dc.subjectApoptosis
dc.subjectApoptosis Regulatory Proteins
dc.subjectCaspase 3
dc.subjectCell Line, Tumor
dc.subjectCell Survival
dc.subjectColonic Neoplasms
dc.subjectDown-Regulation
dc.subjectHT29 Cells
dc.subjectHumans
dc.subjectInflammation
dc.subjectLeuconostoc mesenteroides
dc.subjectMicroRNAs
dc.subjectMitogen-Activated Protein Kinases
dc.subjectNF-kappa B
dc.subjectProto-Oncogene Proteins c-akt
dc.subjectPTEN Phosphohydrolase
dc.subjectSignal Transduction
dc.subjectUp-Regulation
dc.titleLeuconostoc mesenteroides-derived anticancer pharmaceuticals hinder inflammation and cell survival in colon cancer cells by modulating NF-?B/AKT/PTEN/MAPK pathways
dc.typeArticle
dc.citation.volume94
dc.citation.spage1094
dc.citation.epage1100
dc.citation.indexScopus
dc.identifier.DOIhttps://doi.org/10.1016/j.biopha.2017.08.033


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