نمایش پرونده ساده آیتم

dc.contributor.authorHejazi, MS
dc.contributor.authorKhojine, ABS
dc.date.accessioned2018-08-26T08:58:22Z
dc.date.available2018-08-26T08:58:22Z
dc.date.issued2007
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/54685
dc.description.abstractBackground: Knowing that adenoviral vectors could initiate innate immunity, the ability of E1-deleted recombinant adenovirus (Ad-E1?) in induction of B7.1 and IL-2 molecules was studied. Methods: The expression of green fluorescent protein in C1498 cells following transfection of these cells with adenovirus green fluorescent protein vector confirmed the ability of adenovirus vectors in infecting the cells and inducing the expression of the gene of interest. The expression of B7.1 molecule on the surface of the cells was assayed upon infection with Ad-E1? vector. Adenovirus-IL-2/B7.1 vector capable of inducing IL-2 and B7.1 expression in the cells was used as the positive control vector. Results: According to the FACS results, about 4.17% of normal cells expressed B7.1 on their surface, while this level was increased in Ad-E1? transduced cells up to 14.43%. These results demonstrate that Ad-E1? vector considerably (about 3 folds) increases the expression of B7.1 on the cells. No detectable IL-2 was secreted into the medium of non-transduced and Ad-E1? transduced cells. Conclusion: Data indicate that the infection of C1498 cells with recombinant adenoviruses stimulates expression of B7.1 on the cell surface rather than secretion of IL-2 into the medium.
dc.language.isoEnglish
dc.relation.ispartofIranian Biomedical Journal
dc.subjectadenovirus vector
dc.subjectB7 antigen
dc.subjectgreen fluorescent protein
dc.subjectinterleukin 2
dc.subjectrecombinant protein
dc.subjectAdenovirus
dc.subjectanimal cell
dc.subjectarticle
dc.subjectcell line
dc.subjectcell surface
dc.subjectembryo
dc.subjectfluorescence activated cell sorting
dc.subjectgenetic transduction
dc.subjectgenetic transfection
dc.subjecthuman
dc.subjecthuman cell
dc.subjectmouse
dc.subjectnonhuman
dc.subjectprotein expression
dc.subjectvirus infectivity
dc.subjectAdenovirus E1 Proteins
dc.subjectAdenoviruses, Human
dc.subjectAnimals
dc.subjectAntigens, CD80
dc.subjectCell Line
dc.subjectCell Membrane
dc.subjectGene Deletion
dc.subjectGenes, Viral
dc.subjectGenetic Vectors
dc.subjectGreen Fluorescent Proteins
dc.subjectHumans
dc.subjectImmunity, Natural
dc.subjectInterleukin-2
dc.subjectMice
dc.subjectRecombinant Fusion Proteins
dc.subjectTransfection
dc.subjectAdenoviridae
dc.titleInfluence of E1-deleted recombinant adenoviruses on B7.1 and IL-2 expression in C1498 cells
dc.typeArticle
dc.citation.volume11
dc.citation.issue3
dc.citation.spage153
dc.citation.epage160
dc.citation.indexScopus


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