Genetic variations of tumor necrosis factor -α-308 and lymphtoxin-α+252 in non-hodgkin lymphoma and acute lymphoblastic leukemia patients

Abstract

Objective(s): Non- Hodgkin lymphoma (NHL) and acute lymphoblastic leukemia (ALL) are two main hematological malignances which have been driven from lymphoid tissue. Genetic polymorphisms in tumor necrosis factor-? (TNF-?) -308 and lymphotoxin-? (LT-?) +252 may affect their transcription and expression which leads to their high plasma level. The frequency of the TNF-? (-308) and LT-? (+ 252) polymorphisms are different for NHL and ALL cases in various populations with different ethnicity. This research is designed to investigate the prevalence and association of TNF-? (-308) and LT-? (+ 252) polymorphisms from NHL and ALL in Azarian patients and healthy individuals from Northwestern part of Iran. Materials and Methods: Seventy subjects with ALL and 68 NHL, along with another 130 healthy subjects as control group took part in this study. Genomic DNA was extracted, then genetic polymorphisms in TNF-? and LT-? genes were analyzed with the PCR-RFLP and NCOI as restriction enzyme. A statistical analysis was performed by chi-square test using SPSS software. A P -value of <0.05 was considered statistically significant. Results: A statistically significant difference of LT-? polymorphism was in NHL patients and control (P-value= 0.008) but there was not any association of TNF-? polymorphism between NHL patients and control group. A significant association for TNF-a variant was in ALL and control (P-value =0.005), however, there was no relationship about LT variant between ALL and control. Conclusion: The results show that there are significant differences between TNF-? (-308) and LT-? (+252) genetic polymorphisms respectively in ALL and NHL patients with control group from Northwestern part of Iran.