Genetic classification and differentiation of Enterobius vermicularis based on mitochondrial cytochrome c oxidase [cox1) in Northwest of Iran

Abstract

Enterobius vermicularis is one of the most prevalent parasitic helminths, particularly in children. According to the studies, three different genotypes (A, B and C) were revealed from human and chimpanzee. This study conducted to investigate the existence and distribution of different E. vermicularis genotypes based on mitochondrial cytochrome c oxidase subunit 1 (cox1) in northwest of Iran. 45 positive scotch tape samples of E. vermicularis were collected from various areas of Tabriz. After DNA extraction, the targeted DNA region was amplified for the mitochondrial cytochrome c oxidase 1(cox 1) gene by nested PCR method. All amplicons were sequenced and then analyzed by specific phylogenetic software. Results of the present study showed that, Enterobius vermicularis have two subtypes including B1 and B2 in northwest of Iran by Coxl gene sequencing method. In conclusion, B1 and B2 subtypes of Enterobius vermicularis in human are the predominant genotype of this nematode in northwest of Iran. Regarding to the prevalence and public health importance of the disease and role of the parasite genotyping in prevention, control and treatment, further studies are needed to determine genotypes of E. vermicularis in other regions.