Expression Level of miR-34a in Tumor Tissue from Patients with Esophageal Squamous Cell Carcinoma

Abstract

Background: miR-34a has been shown to be involved in P53 regulation. In this study, we aimed to evaluate the expression level of miR-34a in esophageal cancer and compare it with that of the normal marginal tissues. Methods: Tumor and marginal tissues were obtained from 50 patients with esophageal cancer. After RNA extraction, expression level of miR-34a was determined using SYBR green master mix and real-time quantitative PCR. Results: It was observed that there was a downregulation of miR-34a in tumoral tissue of esophageal patients in comparison to normal marginal tissues. Moreover, the expression level of miR-34a was correlated with clinicopathological specifications of the patients. Conclusions: miR-34a may be involved in the pathogenesis and development of esophageal cancer and have the potential to be used as a diagnostic and therapeutic marker in esophageal cancer. é 2018 Springer Science+Business Media, LLC, part of Springer Nature