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dc.contributor.authorEbrahimi-Barough, S
dc.contributor.authorNorouzi Javidan, A
dc.contributor.authorSaberi, H
dc.contributor.authorJoghataei, MT
dc.contributor.authorRahbarghazi, R
dc.contributor.authorMirzaei, E
dc.contributor.authorFaghihi, F
dc.contributor.authorShirian, S
dc.contributor.authorAi, A
dc.contributor.authorAi, J
dc.date.accessioned2018-08-26T08:54:35Z
dc.date.available2018-08-26T08:54:35Z
dc.date.issued2015
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/54165
dc.description.abstractHuman endometrium is a high-dynamic tissue that contains human endometrial stem cells (hEnSCs) which can be differentiated into a number of cell lineages. The differentiation of hEnSCs into many cell lineages such as osteoblast, adipocyte, and neural cells has been investigated previously. However, the differentiation of these stem cells into motor neuron-like cells has not been investigated yet. Different biochemical and topographical cues can affect the differentiation of stem cells into a specific cell. The aim of this study was to investigate the capability of hEnSCs to be differentiated into motor neuron-like cells under biochemical and topographical cues. The biocompatible and biodegradable poly(lactic-co-glycolic acid) (PLGA) electrospun nanofibrous scaffold was used as a topographical cue. Human EnSCs were cultured on the PLGA scaffold and tissue culture polystyrene (TCP), then differentiation of hEnSCs into motor neuron-like cells under induction media including retinoic acid (RA) and sonic hedgehog (Shh) were evaluated for 15آ days. The proliferation rate of cells was assayed by using MTT assay. The morphology of cells was studied by scanning electron microscopy imaging, and the expression of motor neuron-specific markers by real-time PCR and immunocytochemistry. Results showed that survival and differentiation of hEnSCs into motor neuron-like cells on the PLGA scaffold were better than those on the TCP group. Taken together, the results suggest that differentiated hEnSCs on PLGA can provide a suitable, three-dimensional situation for neuronal survival and outgrowth for regeneration of the central nervous system, and these cells may be a potential candidate in cellular therapy for motor neuron diseases. é 2014, Springer Science+Business Media New York.
dc.language.isoEnglish
dc.relation.ispartofMolecular Neurobiology
dc.subjectmolecular scaffold
dc.subjectpolyglactin
dc.subjectpolystyrene
dc.subjectretinoic acid
dc.subjectsonic hedgehog protein
dc.subjectlactic acid
dc.subjectnanofiber
dc.subjectpolyglycolic acid
dc.subjectpolylactic acid-polyglycolic acid copolymer
dc.subjectadipocyte
dc.subjectArticle
dc.subjectbiocompatibility
dc.subjectbiodegradation
dc.subjectcell adhesion
dc.subjectcell differentiation
dc.subjectcell lineage
dc.subjectcell proliferation
dc.subjectcell regeneration
dc.subjectcell structure
dc.subjectcell survival
dc.subjectcell viability
dc.subjectcontrolled study
dc.subjectendometrium cell
dc.subjecthuman
dc.subjecthuman cell
dc.subjectimmunocytochemistry
dc.subjectmotoneuron
dc.subjectMTT assay
dc.subjectnerve cell
dc.subjectosteoblast
dc.subjectreal time polymerase chain reaction
dc.subjectscanning electron microscopy
dc.subjectstem cell
dc.subjectbiodegradable implant
dc.subjectcell culture
dc.subjectcell differentiation
dc.subjectcytology
dc.subjectdrug effects
dc.subjectphysiology
dc.subjectregeneration
dc.subjectstem cell
dc.subjectAbsorbable Implants
dc.subjectCell Differentiation
dc.subjectCell Lineage
dc.subjectCell Proliferation
dc.subjectCell Survival
dc.subjectCells, Cultured
dc.subjectHumans
dc.subjectLactic Acid
dc.subjectMotor Neurons
dc.subjectNanofibers
dc.subjectPolyglycolic Acid
dc.subjectRegeneration
dc.subjectStem Cells
dc.titleEvaluation of Motor Neuron-Like Cell Differentiation of hEnSCs on Biodegradable PLGA Nanofiber Scaffolds
dc.typeArticle
dc.citation.volume52
dc.citation.issue3
dc.citation.spage1704
dc.citation.epage1713
dc.citation.indexScopus
dc.identifier.DOIhttps://doi.org/10.1007/s12035-014-8931-2


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