Evaluation of IL-22 polymorphism in patients with visceral leishmaniasis

Abstract

Objective: To examine the IL-22 gene polymorphism in visceral leishmaniasis (VL) patients in comparison to healthy people as the control group. Methods: The blood samples were collected from 236 people, among them 74 were patients with a history and clinical symptoms of leishmaniasis and 162 were healthy with no signs of infection and all of them were residents in endemic area of VL in East Azerbaijan Province. DNA extraction was performed by using salting out method and amplification refractory mutation system-polymerase chain reaction was used for detecting the polymorphism of IL-22 promoter (rs2227473A/G). Results: In patients group, 9 (12.16%) individuals showed A/A genotype and 29 (39.18%) showed G/G genotype. The heterozygote form (A/G) was 36 (48.64%) among this group. In the control group, the proportions of genotypes were 8.02%, 46.09% and 45.06%, respectively. Conclusions: According to our results, there was no significant association between VL and polymorphism of IL-22 gene promoter (rs2227473A/G). é 2014 Asian Pacific Tropical Medicine Press.