نمایش پرونده ساده آیتم

dc.contributor.authorGhorbian, S
dc.contributor.authorJahanzad, I
dc.contributor.authorJavadi, GR
dc.contributor.authorSakhinia, E
dc.date.accessioned2018-08-26T08:54:15Z
dc.date.available2018-08-26T08:54:15Z
dc.date.issued2014
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/54106
dc.description.abstractPurpose: Evaluation diagnostic usefulness of immunoglobulin light chains (Ig?, Ig?) and incomplete IGH D-J clonal gene rearrangements in formalin-fixed, paraffin-embedded (FFPE) tissue of patients with B-cell non-Hodgkin lymphomas (B-NHL).Materials and methods: This study was performed on samples from 70 patients with B-NHL, including two cases of follicular lymphoma (FL), 20 cases of diffuse large B-cell lymphoma (DLBCL), one case of mantle cell lymphoma (MCL), and 47 cases of B-cell neoplasm (non-classified), which had been previously assessed for complete IGH clonality, and failure to clarify gene rearrangements. We used a gold standard multiplex PCR protocol provided by European Biomedicine and Health (BIOMED-2) Concerted Action Project BMH4-CT98-3936 for improvement of diagnosis and analysis of clonality gene rearrangement in lymphoma malignancies.Results: Our results revealed a total positive monoclonality of 89آ % (62/70) in Ig?, Ig?, and 11.4آ % (8/70) polyclonality in gene rearrangements assay. The samples with positive clonality consisting (Ig?: 45آ %, Ig?: 55آ %) in DLBCL, (Ig?: 100آ %) in FL, (Ig?: 100آ %) in MCL, and (Ig?: 47آ %, Ig?: 36آ %) in B-cell neoplasm non-classified. None of the incomplete IGH D-J immunoglobulin gene families (0آ %) showed monoclonality, and all samples demonstrated polyclonality pattern.Conclusions: Our findings on FFPE tissue revealed that immunoglobulin light chains clonality gene rearrangements assays using BIOMED-2 protocol, could be considered a valuable and reliable method for clonality detection, particularly in cases of failure of complete IGH gene rearrangements analysis. Clonal Ig gene rearrangements assay is applicable for routine diagnostic testing of lymphoproliferative disorders and as a reliable method for differentiating between malignant and benign lymphoma disorders. é 2014, Federaci?n de Sociedades Espa?olas de Oncolog?a (FESEO).
dc.language.isoEnglish
dc.relation.ispartofClinical and Translational Oncology
dc.subjectformaldehyde
dc.subjectimmunoglobulin heavy chain
dc.subjectimmunoglobulin kappa chain
dc.subjectimmunoglobulin lambda chain
dc.subjectincomplete immunoglobulin heavy chain D-J
dc.subjectparaffin
dc.subjectunclassified drug
dc.subjectimmunoglobulin heavy chain
dc.subjectimmunoglobulin light chain
dc.subjecttumor marker
dc.subjectArticle
dc.subjectcancer diagnosis
dc.subjectclinical evaluation
dc.subjectclinical protocol
dc.subjectclonal variation
dc.subjectfemale
dc.subjectfollicular lymphoma
dc.subjectgene rearrangement
dc.subjectgold standard
dc.subjecthuman
dc.subjecthuman tissue
dc.subjectimmunoglobulin structure
dc.subjectlarge cell lymphoma
dc.subjectmajor clinical study
dc.subjectmale
dc.subjectmantle cell lymphoma
dc.subjectmultiplex polymerase chain reaction
dc.subjectreliability
dc.subjectadult
dc.subjectgenetics
dc.subjectLymphoma, B-Cell
dc.subjectAdult
dc.subjectFemale
dc.subjectGene Rearrangement
dc.subjectHumans
dc.subjectImmunoglobulin Heavy Chains
dc.subjectImmunoglobulin Light Chains
dc.subjectLymphoma, B-Cell
dc.subjectMale
dc.subjectMultiplex Polymerase Chain Reaction
dc.subjectTumor Markers, Biological
dc.titleEvaluation diagnostic usefulness of immunoglobulin light chains (Ig?, Ig?) and incomplete IGH D-J clonal gene rearrangements in patients with B-cell non-Hodgkin lymphomas using BIOMED-2 protocol
dc.typeArticle
dc.citation.volume16
dc.citation.issue11
dc.citation.spage1006
dc.citation.epage1011
dc.citation.indexScopus
dc.identifier.DOIhttps://doi.org/10.1007/s12094-014-1188-4


فایلهای درون آیتم

فایلهاسایزفرمتنمایش

هیچ فایل مرتبطی وجود ندارد

این آیتم در مجموعه های زیر مشاهده می شود

نمایش پرونده ساده آیتم