Effect of treadmill exercise and diosgenin on biochemical alterations in isoproterenol-induced myocardial infarction in rats

Abstract

Objectives: Myocardial infarction (MI) is one of the main causes of mortality and morbidity in the world. This study was aimed to evaluate the preventive role of treadmill exercise and Diosgenin on cardiac marker enzymes, lipids and lipoproteins in isoproterenol (ISO)-induced myocardial infarction (MI) in male Wistar rats. Methods: One hundred Wistar rats were divided into ten groups: control rats (C), saline (S), L-Cremephor alone (LC), Diosgenin dissolved in L-Cremephor (15 mg/kg/day) (D), Exercise (E), Exercise + Diosgenin (E+D), ISO injected (150 mg/kg) (ISO), Diosgenin + ISO (D+ISO), Exercise + ISO (E+ISO, and Exercise + Diosgenin + ISO (E+D+ISO). After eight weeks all animals anesthetized and blood samples were collected for biochemical estimation. Results: Exercise and Diosgenin pretreatment decreased significantly the LDH level in ISO injected animals but only exercise can lower CK-MB level. Exercise and Diosgenin pretreatment significantly decreased serum total cholesterol and increased HDL-C. Body weight increased (p<0.05) in E group, but Diosgenin treatment lowered it in all exercise groups. Conclusion: Our findings confirmed the efficacy of exercise and Diosgenin pretreatment on alleviating ISO-induced cardiac damage. This effect may be resulted from positive change of lipid metabolism.