Effect of sodium carboxyl methylcellulose on intestinal permeability of furosemide

Abstract

Objectives: The aim of this project was determination of intestinal absorption of furosemide in the presence of anionic macromolecules. Methods: In the present study the SPIP technique was used for permeability determination in rat intestine. We conduct the experiment on a 10 cm cannulated jejunal segment of anesthetized rats. The segment was filled with perfusion solution. Perfusion rate was 0.2 ml/min and lasted for 90 minutes.Outlet tubing samples were gathered in defined time intervals (every 10 min) and the remaining drug was measured in samples. Samples were analyzed by HPLC system. The calculation of Peff was based on outlet perfusate steady-state concentrations and the initial concentration of drug solution. Results: Obtained data indicated that the intestinal permeability increased by increasing the NaCMC concentration (P <0.001). The increasing could be explained by donnan equilibrium and influence of anionic polymer on tight junctions. Poly electrolytes (like NaCMC) enhance the transport rate of ionic drugs with same electrical charge. Conclusion: Anionic polymers could be used as absorption enhancers for anionic drugs like furosemide in defined concentrations.