Effect of HEMADO on the changes of nitric oxide and coronary flow in isolated hearts of male rats

Abstract

Objectives: The aim of this study is to examine the effects of HEMADO on variations Nitric Oxide and coronary in isolated rat heart. Methods: 21 male Wistar rats (250-300g) divided to four groups, each has seven members (n=7) as follow: without ischemia, control (ischemia without drug), drug (ischemia with drug), and ischemia with ethanol. The animals are anesthetized by Ketamine and Xylazine. The hearts were immediately taken and put on Longendorff apparatus and perfused by Krebs-Henseleit solution under constant pressure and temperature of 37 °C.After 20 minutes of stabilization, Ischemic groups are also received 40 minutes of global ischemia and 90 minutes of reperfusion. In drug group, the hearts were perfused 25 minutes with Hemado-enriched Krebs-Henseleit before the Ischemia. Result: HEMADO significantly enhanced of Nitric Oxide (P <0 / 05), in the drug group compared to the control group but it had no effect on coronary flow. Conclusion: Current study indicated that Hemado could keep the heart against ischemia-reperfusion injury. And it is found that increasing the of Nitric Oxide is responsible for the part of cardioprotective effect of Hemado.