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dc.contributor.authorOmidinia, E
dc.contributor.authorKhanehzar, A
dc.contributor.authorShadjou, N
dc.contributor.authorShahbaz Mohamadi, H
dc.contributor.authorEmami, SH
dc.contributor.authorHasanzadeh, M
dc.date.accessioned2018-08-26T08:51:18Z
dc.date.available2018-08-26T08:51:18Z
dc.date.issued2013
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/53331
dc.description.abstractA new strategy was developed to investigate conducting polymer poly(3,4- ethylenedioxy)thiophene:poly(styrenesulfonate) (PEDOT:PSS) properties and potential for enzymatic electrochemical detection of l-phenylalanine. PEDOT:PSS was blended with polyvinyl alcohol (PVA) and further treated with glutaraldehyde (GA) to produce new conducting polymer composite films of PEDOT:PSS/PVA-GA on indium(tin) oxide (ITO)- coated glass slides. Phenylalanine dehydrogenase (PheDH) was covalently immobilized on the glutaraldehyde modified conducting polymer composite films to produce PEDOT:PSS/PVA-GA-PheDH films. Using this strategy, PEDOT:PSS remained chemically unmodified and its electrical properties was maintained in a matrix containing glutaraldehyde modified polyvinyl alcohol. Electrochemical behavior of the conducting polymer composite films, before and after enzyme immobilization, was investigated using a potentiostat/galvanostat. Cyclic voltammograms showed an oxidation peak of about 0.63 V for PEDOT:PSS/PVA-GA (PEDOT:PSS to PVA ratio=1/1) and about 0.73 V for PEDOT:PSS/PVA-GA-PheDH in different concentrations of L-phenylalanine in Glycine/KCl/KOH buffer solution (100 mM, pH=10.4). The redox properties of PEDOT:PSS/PVA-GA films which is attributed to oxidation of PEDOT seems to contribute to electron transfer to the electrode during enzymatic reaction. Results showed that the system is suitable for determination of L-Phe in human urine for people with phenylketonuria. é 2013 by CEE.
dc.language.isoEnglish
dc.relation.ispartofAnalytical and Bioanalytical Electrochemistry
dc.titleCovalent immobilization of phenylalanine dehydrogenase on glutaraldehydemodified poly (3,4-ethylenedioxy) thiophene: Poly(styrenesulfonate)/ polyvinyl alcohol conducting polymer composite films for electrochemical detection of L-phenylalanine
dc.typeArticle
dc.citation.volume5
dc.citation.issue5
dc.citation.spage597
dc.citation.epage608
dc.citation.indexScopus
dc.citation.URLhttp://abechem.ir/index.php?option=com_content&view=article&id=3&Itemid=3


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