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dc.contributor.authorAsghari, S
dc.contributor.authorKhaniani, MS
dc.contributor.authorDarabi, M
dc.contributor.authorDerakhshan, SM
dc.date.accessioned2018-08-26T08:39:16Z
dc.date.available2018-08-26T08:39:16Z
dc.date.issued2014
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/53011
dc.description.abstractPurpose: Stem cell factor (SCF) plays an important role in the survival, proliferation and differentiation of hematopoietic stem cells and progenitor cells. Potential therapeutic applications of SCF include hematopoietic stem cell mobilization, exvivo stem/progenitor cell expansion, gene therapy, and immunotherapy. Considering the cost and problem in accessibility of this product in Iran, clears the importance of indigenizing production of rhSCF. In the present work, we describe the construction of the soluble rhSCF expression vector in pET-26b (+) with periplasmic localization potential. Methods: Following PCR amplification of human SCF ORF, it is cloned in pET-26b (+) vector in NcoI and XhoI sites. The recombinant construct was transformed into BL21 (DE3) Ecoli strains. Results: The construction of recombinant vector was verified by colony PCR and sequence analysis of pET26b-hSCF vector. Sequence analyses proved that human SCF ORF has been inserted into NcoI and XhoI site with correct orientation downstream of strong T7 promotor and showed no nucleotide errors. Conclusion: The SCF ORF was successfully cloned in pET-26b (+) expression vector and is ready for future production of SCF protein. é 2014 by Tabriz University of Medical Sciences.
dc.language.isoEnglish
dc.relation.ispartofAdvanced Pharmaceutical Bulletin
dc.subjectrecombinant stem cell factor
dc.subjectarticle
dc.subjectcontrolled study
dc.subjectcytoplasm
dc.subjectEscherichia coli
dc.subjectexpression vector
dc.subjectgene insertion
dc.subjectmolecular cloning
dc.subjectnonhuman
dc.subjectnucleotide sequence
dc.subjectopen reading frame
dc.subjectpolymerase chain reaction
dc.subjectsequence analysis
dc.titleCloning of soluble human stem cell factor in pET-26b(+) vector
dc.typeArticle
dc.citation.volume4
dc.citation.issue1
dc.citation.spage91
dc.citation.epage95
dc.citation.indexScopus
dc.identifier.DOIhttps://doi.org/10.5681/apb.2014.014


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