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BACH1 silencing by siRNA inhibits migration of HT-29 colon cancer cells through reduction of metastasis-related genes

dc.contributor.authorDavudian, S
dc.contributor.authorShajari, N
dc.contributor.authorKazemi, T
dc.contributor.authorMansoori, B
dc.contributor.authorSalehi, S
dc.contributor.authorMohammadi, A
dc.contributor.authorShanehbandi, D
dc.contributor.authorShahgoli, VK
dc.contributor.authorAsadi, M
dc.contributor.authorBaradaran, B
dc.date.accessioned2018-08-26T08:36:58Z
dc.date.available2018-08-26T08:36:58Z
dc.date.issued2016
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/52797
dc.description.abstractBackground Metastasis to distant organs is a hallmark of many tumor cells. BACH1 (BTB and CNC homology 1) is a transcriptional factor which promotes the migration and invasion of breast cancer cells. BACH1 expression and its target genes are intimately associated with the metastasis possibility of clinical samples, and BACH1 reduction leads to meaningful depletion in metastasis. The evaluation of BACH1 role in colon cancer remains elusive. This study seeks to further investigate the role of BACH1 in colon cancer cells. Methods Quantitative RT-PCR (qRT-PCR) was used to detect BACH1 expression and other related metastatic genes following siRNA knockdown in colon cancer HT-29 cells. And the protein level assessed by Western blot. MTT assay was to measure the changed cell viability after BACH1 siRNA transfection. Scratch-wound motility assays measured capacity of tumor cell migration of HT-29 cells after BACH1 silencing. Results The inhibitory effect of BACH1 was performed by siRNA knockdown using highly metastatic HT-29 colon cell lines. Quantitative RT-PCR and Western blot analysis revealed that the expression levels of BACH1 mRNA and protein in HT29 cells were significantly suppressed after transfection. Conversely, the BACH1 expression increased migration. Also the CXCR4 and MMP1 expression levels decreased following BACH1 knockdown in HT-29 cells. Conclusion Our results indicated that BACH1 down-regulation in HT29 CRC cells had no effect on cell growth but did inhibit cell migration by decreasing metastasis-related genes expression. Collectively, these results suggest that BACH1 may function as an oncogenic driver in colon cancer and may represent as a potential target of gene therapy for CRC treatment. é 2016 Elsevier Masson SAS
dc.language.isoEnglish
dc.relation.ispartofBiomedicine and Pharmacotherapy
dc.subjectchemokine receptor CXCR4
dc.subjectcollagenase 3
dc.subjectgelatinase B
dc.subjecthigh mobility group A2 protein
dc.subjectinterstitial collagenase
dc.subjectmicroRNA 34a
dc.subjectsmall interfering RNA
dc.subjecttranscription factor
dc.subjecttranscription factor bach1
dc.subjecttranscription factor Snail
dc.subjecttranscription factor snail1
dc.subjectunclassified drug
dc.subjectBACH1 protein, human
dc.subjectbasic leucine zipper transcription factor
dc.subjectchemokine receptor CXCR4
dc.subjectCXCR4 protein, human
dc.subjectFanconi anemia protein
dc.subjecthigh mobility group A2 protein
dc.subjectinterstitial collagenase
dc.subjectMMP1 protein, human
dc.subjectsmall interfering RNA
dc.subjectSNAI1 protein, human
dc.subjecttranscription factor Snail
dc.subjectantineoplastic activity
dc.subjectArticle
dc.subjectbreast cancer
dc.subjectcancer cell
dc.subjectcancer gene therapy
dc.subjectcancer inhibition
dc.subjectcancer patient
dc.subjectcancer staging
dc.subjectcell growth
dc.subjectcell migration
dc.subjectcell viability
dc.subjectcolon cancer
dc.subjectdisease association
dc.subjectdown regulation
dc.subjectgene expression
dc.subjectgene silencing
dc.subjectgene targeting
dc.subjectgenetic transfection
dc.subjectHT 29 cell line
dc.subjecthuman
dc.subjecthuman cell
dc.subjecthuman tissue
dc.subjectMTT assay
dc.subjectpriority journal
dc.subjectreverse transcription polymerase chain reaction
dc.subjectRNA interference
dc.subjectupregulation
dc.subjectWestern blotting
dc.subjectcell motion
dc.subjectcell survival
dc.subjectcolon tumor
dc.subjectgene expression regulation
dc.subjectgenetics
dc.subjectHT-29 cell line
dc.subjectmetabolism
dc.subjectmetastasis
dc.subjectpathology
dc.subjectRNA interference
dc.subjectsignal transduction
dc.subjecttime factor
dc.subjectBasic-Leucine Zipper Transcription Factors
dc.subjectBlotting, Western
dc.subjectCell Movement
dc.subjectCell Survival
dc.subjectColonic Neoplasms
dc.subjectFanconi Anemia Complementation Group Proteins
dc.subjectGene Expression Regulation, Neoplastic
dc.subjectHMGA2 Protein
dc.subjectHT29 Cells
dc.subjectHumans
dc.subjectMatrix Metalloproteinase 1
dc.subjectNeoplasm Metastasis
dc.subjectReceptors, CXCR4
dc.subjectReverse Transcriptase Polymerase Chain Reaction
dc.subjectRNA Interference
dc.subjectRNA, Small Interfering
dc.subjectSignal Transduction
dc.subjectSnail Family Transcription Factors
dc.subjectTime Factors
dc.subjectTransfection
dc.titleBACH1 silencing by siRNA inhibits migration of HT-29 colon cancer cells through reduction of metastasis-related genes
dc.typeArticle
dc.citation.volume84
dc.citation.spage191
dc.citation.epage198
dc.citation.indexScopus
dc.identifier.DOIhttps://doi.org/10.1016/j.biopha.2016.09.021


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