Alishewanella tabrizica sp. nov., isolated from qurug?l lake

Abstract

A novel Gram-negative, aerobic, motile and rod-shaped bacterium was isolated from Qurug?l Lake located in a mountainous region near Tabriz city in the north-west of Iran. Growth occurred at pH 6-10 (optimum, pH 7آ±0.5) and at 10-40آ°C (optimum, 30آ°C). Strain RCRI4T was able to grow in the absence and presence of NaCl to 3% (w/v). The major fatty acids were C17:0 C16: 1? 7c/C15 iso3-OH1 C17:1 ? 8c and C16:0. The G+C content of genomic DNA was 45.3 mol%. Based on the 16S rRNA and gyrB gene sequences, phylogenetic analyses indicated that strain RCRI4T associated with the genus Alishewanella, and closely related type strains include Alishewanella agri BLO6T (97.8 %), Alishewanella aestuarii B11T (97.7 %), Rheinheimera perlucida BA131T (97.5 %), Alishewanella fetalis CCUG 30811T (97.3 %) and Alishewanella jeotgali MS1T (97.1 %). The level of DNA-DNA relatedness between strain RCRI4T and phylogenetically the closest related strains, A. agri BLO6T and R. perlucida BA131T, was 9 and 14 %, respectively. On the basis of phenotypic, chemotaxonomic and phylogenetic results, it is suggested that strain RCRI4T represents a novel species of the genus Alishewanella, for which the name Alishewanella tabrizica sp. nov. is proposed. The type strain is RCRI4T (=LMG 26473T=JCM 1727T=KCTC 23723T).é 2012 IUMS.