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dc.contributor.authorSaieed, P|| Reza, RM|| Abbas, D|| Seyyedvali, R|| Aliaschar, H
dc.date.accessioned2018-08-26T08:30:18Z
dc.date.available2018-08-26T08:30:18Z
dc.date.issued2006
dc.identifier10.1248/cpb.54.9
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/51954
dc.description.abstractRuta graveolens L. is a flavonoid-containing medicinal plant with various biological properties. In the present study, the effects of R. graveolens extract on aldehyde oxidase, a molybdenum hydroxylase, are investigated. Aldehyde oxidase was partially purified from liver homogenates of mature male guinea pigs by heat treatment and ammonium sulphate precipitation. The total extract was obtained by macerating the aerial parts of R. graveolens in MeOH 70% and the effect of this extract on the enzyme activity was assayed using phenanthridine, vanillin and benzaldehyde as substrates. Quercetin and its glycoside form, rutin were isolated, purified and identified from the extract and their inhibitory effects on the enzyme were investigated. R. graveolens extract exhibited a high inhibition on aldehyde oxidase activity (89-96%) at 100 mu g/ml which was comparable with 10 mu m of menadione, a specific potent inhibitor of aldehyde oxidase. The IC50 values for the inhibitory effect of extract against the oxidation of benzaldehyde, vanillin and phenanthridine were 10.4, 10.1, 43.2 mu g/ml, respectively. Both quercetin and rutin at 10 mu m caused 70-96% and 27-52% inhibition on the enzyme activity, respectively. Quercetin was more potent inhibitor than rutin, but both flavonols exerted their inhibitory effects mostly in a linear mixed-type.
dc.language.isoEnglish
dc.relation.ispartofCHEMICAL & PHARMACEUTICAL BULLETIN
dc.subjectRuta graveolens L.|| aldehyde oxidase|| flavonoid|| quercetin|| rutin
dc.titleInhibitory effects of Ruta graveolens L. extract on guinea pig liver aidehyde oxidase
dc.typeArticle
dc.citation.volume54
dc.citation.issue1
dc.citation.spage9
dc.citation.epage13
dc.citation.indexWeb of science


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