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dc.contributor.authorSorouraddin, MH
dc.contributor.authorAmini, K
dc.contributor.authorNaseri, A
dc.contributor.authorRashidi, MR
dc.date.accessioned2018-08-26T08:12:25Z
dc.date.available2018-08-26T08:12:25Z
dc.date.issued2010
dc.identifier10.2478/s11532-009-0121-0
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/50772
dc.description.abstractThe multivariate calibration methods, partial least squares (PLS) and principle component regression (PCR) have been used to determine phenanthridine, phenanthridinone and phenanthridine N-oxide in spiked human plasma samples. Resolution of binary and ternary mixtures of analytes with minimum sample pre-treatment and without analyte separation has been successfully achieved analyzing the UV spectral data. The net analyte signal (NAS) concept was also used to calculate multivariate analytical figures of merit such as limit of detection, selectivity and sensitivity. The simultaneous determination of three analytes was possible by PLS and PCR processing of sample absorbance in the 210-355 nm region. Good recoveries were obtained for both synthetic mixtures and spiked human plasma samples.
dc.language.isoEnglish
dc.relation.ispartofCENTRAL EUROPEAN JOURNAL OF CHEMISTRY
dc.subjectMultivariate calibration
dc.subjectSpectrophotometry
dc.subjectPhenanthridine
dc.subjectPhenanthridinone
dc.subjectPhenanthridine N-oxide
dc.titleSimultaneous spectrophotometric determination of phenanthridine, phenanthridinone and phenanthridine N-oxide using multivariate calibration methods
dc.typeArticle
dc.citation.volume8
dc.citation.issue1
dc.citation.spage207
dc.citation.epage213
dc.citation.indexWeb of science
dc.identifier.DOIhttps://doi.org/10.2478/s11532-009-0121-0


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