In Vitro Studies on Calf Thymus DNA interaction with Quercetin-Palladium (II) Complex

Abstract

Flavonoids are antioxidants, which are known to influence bioavailability of the metal in the body. Small organic molecules binding to nucleic acids are of great interest in modern medicine because they constitute a significant portion of the anticancer drugs. Binding studies of flavonoids with DNA are useful for the understanding of the reaction mechanism and providing guidance for the application and design of new and more efficient drugs targeted to DNA. Quercetin one of the most abundant dietary flavonoids, has been investigated in the presence of Pd (II) in 50% Ethanol. Structure of the complex was determined through UV-Vis studies. Binding interaction of this complex with calf thymus (CT) DNA has been investigated by Absorption spectroscopy. The binding constants (K-b) for complex with DNA were estimated to be 4.38x10(3), through spectroscopic titrations. The complex displays significant binding properties to the CT-DNA. The experimental results suggest that Quercetin Pd(II) could bind to DNA via outside binding mode.