Immunohistochemical Study of the Effect of Resveratrol on the Expression of beta-Catenin Protein in Experimental Colonic Carcinoma of Rat

Abstract

Colorectal cancer is a major health problem worldwide. Resveratrol is a stilbenoid, a type of natural phenol and a phytoalexin produced naturally by several plants when under attack by pathogens such as bacteria or fungi. The main objective of present study was to immunohistochemical assessment of the effect of resveratrol on the expression of beta-catenin protein in experimental colonic carcinoma of rat. The 25 male Wistar rats aged 3-4 months old weighting 250-350 g were selected by chance. The rats of group 1 were received standard food and water without any changes in. their nutritional condition. Rats of group 2 were received EDTA at the dose of 40 mg kg(-1) as promotor of DMH twice a week for 2 weeks. Rats of group 3 were received DMH at the dose of 40 mg kg(-1) twice a week for 2 weeks for induction the cancer. The rats of groups 4 and 5 after induction of cancer were received resveratrol at the dose of 10 and 20 mg/kg/day for 10 weeks orally, respectively. The 12 weeks after treatment with resveratrol, animals were constrained and anesthetized by xylazine and ketamine intraperitoneally. Then, segments of colon were sampled for histopathological assessments. Immunohistochemical evaluations showed that rate of the expression of beta-catenin proteins in treatment groups was less than control group and there is a statistical significance among groups (p<0.01).