Genetic Variations of Tumor Necrosis Factor -alpha-308 and Lymphtoxin-alpha+252 in Non-Hodgkin Lymphoma and Acute Lymphoblastic Leukemia Patients

Abstract

Objective(s): Non-Hodgkin lymphoma (NHL) and acute lymphoblastic leukemia (ALL) are two main hematological malignances which have been driven from lymphoid tissue. Genetic polymorphisms in tumor necrosis factor-alpha (TNF-alpha) -308 and lymphotoxin-alpha (LT-alpha) + 252 may affect their transcription and expression which leads to their high plasma level. The frequency of the TNF-alpha (-308) and LT-alpha (+ 252) polymorphisms are different for NHL and ALL cases in various populations with different ethnicity. This research is designed to investigate the prevalence and association of TNF-alpha (-308) and LT-alpha (+ 252) polymorphisms from NHL and ALL in Azarian patients and healthy individuals from Northwestern part of Iran. Materials and Methods: Seventy subjects with ALL and 68 NHL, along with another 130 healthy subjects as control group took part in this study. Genomic DNA was extracted, then genetic polymorphisms in TNF-alpha and LT-alpha genes were analyzed with the PCR-RFLP and NCOI as restriction enzyme. A statistical analysis was performed by chi-square test using SPSS software. A P-value of < 0.05 was considered statistically significant. Results: A statistically significant difference of LT-alpha polymorphism was in NHL patients and control (P-value=0.008) but there was not any association of TNF-alpha polymorphism between NHL patients and control group. A significant association for TNF-alpha variant was in ALL and control (P-value=0.005), however, there was no relationship about LT variant between ALL and control. Conclusion: The results show that there are significant differences between TNF-alpha (-308) and LT-alpha (+ 252) genetic polymorphisms respectively in ALL and NHL patients with control group from Northwestern part of Iran.