A dispersive liquid-liquid microextraction and chiral separation of carvedilol in human plasma using capillary electrophoresis

Abstract

Background: Development of simple, rapid and precise analysis of chiral drugs in biological samples is an important issue. Dispersive liquid-liquid microextraction in combination with CE using field amplified sample injection has been of interest because of its capability to analyze trace amount of drugs. Methods: Dispersive liquid-liquid microextraction-CE-field amplified sample injection was employed for chiral separation of carvedilol in human plasma using UV-DAD detector and the developed method has been validated according to US FDA method validation guideline for bioanalysis. Results: The method was linear over a concentration range of 12.5-100 ng/ml for each carvedilol enantiomer (R-2 = 0.998) and the mean recoveries ranged from 91 to 107%. Conclusion: The method was adapted for sensitive, selective and rapid determination of carvedilol enantiomers in human plasma samples.