Matrix gamma-carboxyglutamate protein and Fetuin-A, in wet type age-related macular degeneration

Abstract

AIM: To evaluate the high sensitivity C-reactive protein (hsCRP), Fetuin -A and matrix gamma -carboxyglutamate protein (MGP) as the main factors for vascular calcification and inflammation in serum of patients with advanced age related macular degeneration (ARMD) in comparison to healthy controls. METHODS: The subjects were 40 patients with choroidal neovascularization (CNV) having a mean age of 70.9 +/- 9.1y and a matched group of 49 apparently healthy control subjects. The ARMD was diagnosed using a slit-lamp with superfield lens, fundus photography and fluorescein angiography. Measurement of hsCRP was done by nephelometry method. Levels of Fetuin-A and MGP were measured by enzyme-linked immunosorbent assay (ELISA) technique. RESULTS: hsCRP [0.45(0.07-2.63) mg/L vs 0.25(0.03-1.2) mg/L, P=0.02)] and Fetuin-A levels (50.27 +/- 5.04 vs 44.99 +/- 10.28 ng/mL, P=0.009) were higher in the patients than in the control groups. We could not find significant difference in MGP level between two groups (P=0.08). There was not a significant correlation between MGP with Fetuin A and hsCRP among the patients (P=0.7, P=0.9 respectively). A significant negative correlation of hsCRP with Fetuin A was observed in both case and control groups (P=0.004, r=-0.33 and P=0.001, r =-0.54, respectively). CONCLUSION: Although our study shows that serum hsCRP and Fetuin A is increased in CNV patients as well as negatively correlated with both study groups, their direct role on pathogenesis of ARMD required future studies.