Fabrication of biodendrimeric beta-cyclodextrin via click reaction with potency of anticancer drug delivery agent

Abstract

The aim of this work was the synthesis of biodendrimeric beta-cyclodextrin (beta-CD) on the secondary face with encapsulation efficacy, with beta-CDs moiety to preserve the biocompatibility properties, also particularly growth their loading capacity for drugs with certain size. The new dendrimer, having 14 beta-CD residues attached to the core beta-CD in secondary face (11), was prepared through click reaction. The encapsulation property of the prepared compound was evaluated by methotrexate (MTX) drug molecule. Characterization of compound 11 was performed with H-1 NMR, C-13 NMR and FTIR and its supramolecular inclusion complex structure was determined using FTIR, DLS, DSC and SEM techniques. In vitro cytotoxicity test results showed that compound 11 has very low or no cytotoxic effect on T47D cancer cells. In vitro drug release study at pHs 3, 5 and 7.4 showed that the release process was noticeably pH dependent and the dendrimer could be used as an appropriate controlled drug delivery system (DDS) for cancer treatment. (C) 2015 Elsevier B.V. All rights reserved.