Aflatoxin B-1 in eggs and chicken livers by dispersive liquid-liquid microextraction and HPLC

Abstract

A rapid, low-cost and simple technique has been developed for the determination of aflatoxin B-1 (AFB(1)) in eggs and livers using high-performance liquid chromatography (HPLC) with UV detection. In this study, the presence of AFB(1) was investigated in 150 eggs and 50 chicken livers from the local market of Tabriz, Iran. AFB(1) was extracted with a mixture of acetonitrile:water (80:20) and cleaned up by dispersive liquid-liquid microextraction which is a very economical, fast and sensitive method. AFB(1) was quantified by HPLC-UV without need for any complex derivatisation in samples to enhance the detection. The results showed that 72% of the liver and 58% of the egg samples were contaminated with AFB(1) ranging from 0.30 to 16.36 mu gkg (-1). limit of detection and limit of quantification for AFB(1) were 0.08 and 0.28 mu gkg (- 1), respectively. The proposed method is suitable for fast analysing of AFB(1) in egg and liver samples.