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dc.contributor.authorAlizadeh, AA
dc.contributor.authorHamzeh-Mivehroud, M
dc.contributor.authorDastmalchi, S
dc.date.accessioned2018-08-26T07:41:30Z
dc.date.available2018-08-26T07:41:30Z
dc.date.issued2015
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/47559
dc.description.abstractPurpose: Tumor necrosis factor alpha (TNF-alpha) is an inflammatory cytokine, involved in both physiological and pathological pathways. Because of central role of TNF-alpha in pathogenesis of inflammatory diseases, in the current study, we aimed to identify novel scFv antibodies against TNF-alpha using phage display technology. Methods: Using libraries composed of phagemid displaying scFv antibodies, four rounds of biopanning against TNF-alpha were carried out, which led to identification of scFvs capable of binding to TNF-alpha. The scFv antibody with appropriate binding affinity towards TNF-alpha, was amplified and used in ELISA experiment. Results: Titration of phage achieved from different rounds of biopanning showed an enrichment of specific anti-TNF-alpha phages during biopanning process. Using ELISA experiment, a binding constant (Kd) of 1.11 +/- 0.32 nM was determined for the phage displaying J48 scFv antibody. Conclusion: The findings in the current work revealed that the identified novel scFv antibody displayed at the N-terminal of minor coat proteins of phagemid binds TNF-alpha with suitable affinity. However, the soluble form of the antibody is needed to be produced and evaluated in more details regarding its binding properties to TNF-alpha.
dc.language.isoEnglish
dc.relation.ispartofADVANCED PHARMACEUTICAL BULLETIN
dc.subjectTNF-alpha
dc.subjectPhage display
dc.subjectSingle chain variable fragment
dc.subjectAntibody library
dc.titleIdentification of Novel Single Chain Fragment Variable Antibodies Against TNF-alpha Using Phage Display Technology
dc.typeArticle
dc.citation.volume5
dc.citation.spage661
dc.citation.epage666
dc.citation.indexWeb of science
dc.identifier.DOIhttps://doi.org/10.15171/apb.2015.090


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