Pharmacological and histological examination of atorvastatin-PVP K30 solid dispersions

Abstract

The objective of the present study was to investigate the pharmacological efficiency of orally administered atorvastatin calcium (ATV) solid dispersions (SDs). SDs of ATV were prepared using polyvinylpyrrolidone K30 (PVP) through the solvent evaporation method. Physicochemical characteristics of the prepared formulations were assessed benefitting scanning electron microscopy (SEM), differential scanning calorimetty (DSC) and powder X-ray diffractometry (PXRD). The drug dissolution rates (under sink and non-sink conditions) as well as solubility studies were also examined. Serum lipid levels, liver index and histological analysis of the liver tissue in hyperlipidemic rats were considered to evaluate the pharmacological efficiency of prepared SDs. According to our findings, the drug crystallinity was reduced and the drug dissolution characteristics were improved in the prepared SDs. In vivo studies revealed that oral administration of ATV (3 mg/kg/day) in the SD form (ASDT) for 14 days along with high fat diet (HFD) to the hypercholesterolemic rats led to a significant decline (P < 0.05) in serum level of total cholesterol (TC) and low density lipoprotein-cholesterol (LDL-C). Moreover, ASDT exhibited more beneficial effects on the liver steatosis compared to ATV physical mixture (APMT) and hyperlipidemic control (HC) groups. In the present study, it was conduded that the SDs of ATV with improved physicochemical characteristics provided an increased therapeutic potential for management of hyperlipidemia compared to the corresponding physical mixtures (PMs). (C) 2015 Elsevier B.V. All rights reserved.