Isolation and Identification of Bacteriophage Effective on Carbapenemase-resistant Pseudomonas aeruginosa Strains

Abstract

Aims and Objectives: Pseudomonas aeruginosa is one of the bacteria which exist in oral cavity sores and is challenging for oral diseases specialists. The development of resistance to antibiotics has caused researchers to think of using new treatment approaches such as phage therapy. This study aims to identify and isolate the bacteriophage that is effective on P. aeruginosa causing oral infections. The present research is a laboratory study on six Pseudomonas spp. strains with different carbapenemase-resistant genes. Materials and Methods: Specific phages were isolated from enriched sludge (microbial pentavalent compound). After isolation, to examine the physical and biological structure of bacteriophage, uracil negative staining protocol was used, and finally, the structure was observed using an electron microscope. Antimicrobial effects were evaluated by double layer and spot method. Results: Two types of bacteriophages from Plasmaviridae and Inoviridae families were identified. Isolated phages had the physical and biological structure of bacteriophage and had bactericidal effects against Pseudomonas spp. isolates harboring SPM gene and multi-drug resistant isolates. Conclusion: Findings of this study indicate the usefulness of bacteriophages for controlling carbapenemase resistant isolates. They can be used in combination with other therapies such as antibiotics or mouth washes especially in deep infectious areas such as mouth ulcers.