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dc.contributor.authorYavari, R
dc.contributor.authorBadalzadeh, R
dc.contributor.authorAlipour, MR
dc.contributor.authorTabatabaei, SM
dc.date.accessioned2018-08-26T07:24:02Z
dc.date.available2018-08-26T07:24:02Z
dc.date.issued2016
dc.identifier10.4103/0253-7613.194847
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/46488
dc.description.abstractObjectives: Inflammation plays a critical role in the progression of diabetic complications such as neurological disorders. Previous reports have indicated the memory-improving effect of troxerutin, in rat hippocampus, but the underlying mechanisms are unclear. Hence, we have investigated the effect of troxerutin pretreatment on gene expressions of inflammation-related microRNAs (miRs), miR-146a and miR-155, and nuclear factor-kappa B (NF-B) signaling pathway in the hippocampus of healthy and diabetic rats. Materials and Methods: Wistar rats were randomly divided into four groups (control, control + troxerutin, diabetic, and diabetic + troxerutin). Diabetes was induced by a single i.p. injection of streptozotocin (50 mg/kg). Troxerutin (150 mg/kg) was orally administered in animals for 1 month. After 10 weeks of diabetes, animals were anesthetized and decapitated for the isolation of hippocampus. The expression of miR-146a and miR-155 and the messenger RNA (mRNA) expressions of NF-B, interleukin-1 receptor-associated kinase-1 (IRAK-1), and tumor necrosis factor receptor-associated factor-6 (TRAF-6) were analyzed by real-time polymerase chain reaction. Results: Diabetes significantly increased hippocampal mRNA levels of NF-B, IRAK-1, and TRAF-6 compared with nondiabetic rats (P < 0.05); however, pretreatment with troxerutin decreased them in both diabetic and nondiabetic animals, independent of its glycemic effect (P < 0.05). The expression levels of miR-146a and miR-155 were decreased in diabetic group as compared to the control (P < 0.01). Conclusion: These findings showed that troxerutin could inhibit the inflammatory NF-B pathway in the hippocampus of diabetic rats, which may be due to the negative feedback loop regulated by miR-146a.
dc.language.isoEnglish
dc.relation.ispartofINDIAN JOURNAL OF PHARMACOLOGY
dc.subjectDiabetes
dc.subjectinflammation
dc.subjectmicroRNA-146a
dc.subjectnuclear factor-kappa B
dc.subjecttroxerutin
dc.titleModulation of hippocampal gene expression of microRNA-146a/microRNA-155-nuclear factor-kappa B inflammatory signaling by troxerutin in healthy and diabetic rats
dc.typeArticle
dc.citation.volume48
dc.citation.issue6
dc.citation.spage675
dc.citation.epage680
dc.citation.indexWeb of science


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