| dc.description.abstract | Background: Some medicinal plants with their antioxidant properties have protective effects on sperm parameters and neutralize the harmful effects of lead exposure. Objectives: The present study aimed to determine the effects of Melissa on sperm parameters and spermatogenesis quality in adult rats exposed to lead. Methods: This animal experimental study was conducted on 45 male Wistar rats weighing 180 +/- 20 g, which were allocated randomly into five groups (n=9): distilled water gavages (control) (0.5 ml); intraperitoneal distilled water (i.p. control) (0.5 ml); Melissa (100 mg/kg/bw); lead acetate (10 mg/kg/bw); and lead acetate (10 mg/kg/bw) plus Melissa (100 mg/kg/bw). After a 30-day treatment, the animals were sacrificed. Sperm parameters (count, motility, morphology, and chromatins assay) and weighing of the epididymis and testis were performed. Results: The results showed that in the lead acetate plus Melissa group, epididymis weight (0.37gr vs. 0.32gr), testis weight (1.40 vs. 1.28g), sperm motility (4.44% vs. 33.2 %(, and viable sperm (90 vs. 50) were higher than the lead group. However, testicular weight, normal morphology, and DNA percentage showed insignificant differences among the study groups (P > 0.05). Conclusions: The results of the present study indicate the protective effects of Melissa officinalis on some sperm parameters and spermatogenesis quality in rats exposed to lead. | |
