Supramolecular encapsulation of doxorubicin with beta-cyclodextrin dendrimer: in vitro evaluation of controlled release and cytotoxicity

Abstract

In this study, the formation of supramolecular inclusion complex of doxorubicin (DOX), a high loading and pH-dependent delivery of DOX on beta-CD dendrimer was studied. beta-cyclodextrin (beta-CD) dendrimer having beta-CD in both periphery and core was prepared with entrapment efficiency using click reaction. The encapsulation property of the beta-CD-dendrimer was investigated by DOX as model drug. The chemical construction of beta-CD-dendrimer was described by H-1 NMR, C-13 NMR and FTIR and its inclusion complex construction was studied by FTIR, DSC, SEM, and DLS techniques. It was confirmed that beta-CD dendrimer able to encapsulate DOX in solution; as a result, the designed complex revealed pH-dependent sustained release of DOX, in vitro. Also, the in vitro outcomes on T47D cells displayed that complexation of DOX with beta-CD dendrimer involved an improvement of in vitro cytotoxicity and anticancer activity and this data appeared to be as a result of the developed solubility of the DOX.