| dc.description.abstract | Hydroxylapatite (HA) and sodium fluoride (NaF) are expected to have enhanced osteoblast proliferation and differentiation ability and as a result gained much attention in tissue-engineering applications. The aim of this study is to investigate the effect of NaFand/or HA addition to three-dimensional (3D) nanofibrous poly(s-caprolactone) (PCL)/poly lactic acide (PLA) scaffolds on Human dental pulp stem cells (DPSCs) proliferation, osteoblast differentiation and mineralization. For this purpose, PCL and PLA homopolymers and HA nanoparticles were synthesized. Then a 3D nanofibrous composite scaffold of PCL/PLA/HA was prepared by electrospinning method with fiber diameter in the range of around 120-300 nm. NaF was added to media during DPSCs culturing step. Cell proliferation was assessed over a 14 days period. Reverse transcription polymerase chain reaction (RT-PCR) was used to evaluate the osteogenic induction. The in vitro biocompatibility tests performed with DPSCs showed excellent cell attachment (SEM), proliferation (MTT assay), and mineralization (Alizarin Red). QRT-PCR results, demonstrated significantly superior osteogenesis markers in group of DPSCs bioengineered in PCL/PLA/HA and PCL/PLA/HA/NaF. Simultaneous application of HA in nanofibrous PCL/PLA scaffolds and NaF in the media showed synergistic effects on the growth and differentiation of DPSCs, and consequently seems to be a promising scaffold for tissue regeneration in the oral cavity. | |
