| dc.description.abstract | The presence of foodborne pathogens is a major concern for the food industry and increase in antibiotic resistance adds to the seriousness of this issue. Epidemiological studies have shown that there is little or no information from Iran on the prevalence of Campylobacter spp. in chickens of slaughter age. The aim of this study was to determine the prevalence, antibacterial susceptibility and type of Campylobacter species isolated from the cecum of chickens bred in Saqqez city, Kurdistan, western Iran. Campylobacter was isolated and identified by culture and molecular methods. Antibiotic susceptibility of Campylobacter species was performed by disk agar diffusion test and agar dilution methods. The bacterial isolates were typed by repetitive element sequence based polymerase chain reaction (rep-PCR) method. Fifty-five percent of the farms were found to be contaminated with Campylobacter spp. Gene amplification assay confirmed 67 isolates with Campylobacter spp., of which 57 (85.1%) were identified as C. jejuni and 10 (14.9%) as C. coli. Resistance to tetracycline was the most common finding (70.6%), followed by ciprofloxacin (63.7%) and amoxicillin (27.5%). All isolates retained their susceptibility towards gentamicin and meropenem. Results of MIC50 and MIC90 confirmed high resistance towards tetracycline and ciprofloxacin. Repetitive element sequence based-polymerase chain reaction (rep-PCR) placed C. jejuni in six profiles, while C. coli could not be separated as diverse clones. The present study focused on obtaining data regarding prevalence, antibiotic susceptibilities, genetic diversity at regular intervals and maintain and improve hygiene. The results of this study showed substantial genetic diversity of C. jejuni in chickens from western Iran. | |
