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dc.contributor.authorMehdipour, M
dc.contributor.authorKia, HD
dc.contributor.authorMoghaddam, G
dc.contributor.authorHamishehkar, H
dc.date.accessioned2018-08-26T06:35:07Z
dc.date.available2018-08-26T06:35:07Z
dc.date.issued2018
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/44141
dc.description.abstractThis experiment was conducted to study the effects of egg yolk plasma (10%, 15% and 20%), soybean lecithin (0.5%, 1% and 1.5%) and whole egg yolk (WEY) (control) on post-thawed sperm quality, hatchability and fertility outcomes. In experiment 1, sperm motility, abnormalities, membrane integrity, viability, apoptosis status, mitochondrial activity were studied following freeze-thawing. The best quality of frozen-thawed rooster sperm was chosen to be used for the assessment of the hatchability and fertility rate in experiment 2. The significantly higher percentages of post-thawing sperm total and progressive sperm motilities, membrane integrity, viability were observed in 1% soybean lecithin and 20% egg yolk plasma in comparison with 0.5 and 1% soybean lecithin, 10% egg yolk plasma and control, except for 15% egg yolk plasma (P < 0.05). Using 20% egg yolk plasma in the extender improved mitochondrial activity. Supplementation of 1% soybean lecithin and 20% egg yolk plasma into the extender resulted in the least percentages of dead sperm (P < 0.05). Sperm abnormalities and early apoptosis did not differ in various extender supplementations. In experiment 2, higher percentages of hatchability and fertility rate were observed in semen containing 1% soybean lecithin and 20% egg yolk plasma compared with the WEY group. The results showed that supplementation of the rooster sperm extender with 1% soybean lecithin and 20% egg yolk plasma resulted in higher quality of frozen-thawed sperm. (C) 2018 Elsevier Inc. All rights reserved.
dc.language.isoEnglish
dc.relation.ispartofTHERIOGENOLOGY
dc.subjectFreezing
dc.subjectRooster semen
dc.subjectArtificial insemination
dc.subjectCryoprotectant
dc.titleEffect of egg yolk plasma and soybean lecithin on rooster frozen-thawed sperm quality and fertility
dc.typeArticle
dc.citation.volume116
dc.citation.spage89
dc.citation.epage94
dc.citation.indexWeb of science
dc.identifier.DOIhttps://doi.org/10.1016/j.theriogenology.2018.05.013


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