Formulation, characterization and cytotoxicity evaluation of ketotifen-loaded nanostructured lipid carriers

Abstract

Ketotifen (KT) as a second generation H1-antihistamine is used to relieve allergic conjunctivitis and allergies. To improve KT systemic bioavailability and reduce cellular toxicity, it was formulated into nanostructured lipid carriers (NLCs). KT-loaded NLCs were prepared using hot homogenization and ultrasonication methods and characterized using Zetasizer and transmission electron microscopy (TEM). The particle size and encapsulation efficiency (EE) of KT-loaded NLCs were around 100 nm and 70%, respectively. TEM image of NLCs revealed spherical shaped particles and verified the Zetasizer results. MTT and DAPI staining assays confirmed the low toxicity of KT-loaded NLCs. Finally, the prepared delivery system can be a surrogate for the traditionally available products used in the treatment of allergic symptoms.