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dc.contributor.authorHasanzadeh, M
dc.contributor.authorZargami, A
dc.contributor.authorBaghban, HN
dc.contributor.authorMokhtarzadeh, A
dc.contributor.authorShadjou, N
dc.contributor.authorMahboob, S
dc.date.accessioned2018-08-26T06:34:48Z
dc.date.available2018-08-26T06:34:48Z
dc.date.issued2018
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/44071
dc.description.abstractThe genetic disorder phenylketonuria (PKU) is the inability to metabolize phenylalanine because of a lack of the enzyme phenylalanine hydroxylase. Phenylalanine is used to biochemically form proteins, coded for by DNA. The development of an apta-assay for detection of L-Phenylalanine is presented in this work. A highly specific DNA-aptamer, selected to L-Phenylalanine was immobilized onto a gold nanostructure and electrochemical measurements were performed in a solution containing the phosphate buffer solution with physiological pH. We have constructed an aptamer immobilized gold nanostructure mediated, ultrasensitive electrochemical biosensor (Apt/AuNSs/Au electrode) for L-Phenylalanine detection without any additional signal amplification strategy. The aptamer assemble onto the AuNSs makes Apt/AuNSs/Au electrode an excellent platform for the L-Phenylalanine detection in physiological like condition. Differential pulse voltammetry were used for the quantitative L-Phenylalanine detection. The Apt/AuNSs/Au electrode offers an ultrasensitive and selective detection of L-Phenylalanine down to 0.23 mu M level with a wide dynamic range from 0.72 mu M-6 mM. The aptasensor exhibited excellent selectivity and stability. The real sample analysis was performed by spiking the unprocessed human serum samples with various concentration of L-Phenylalanine and obtained recovery within 2% error value. The sensor is found to be more sensitive than most of the literature reports. The simple and easy way of construction of this apta-assay provides an efficient and promising diagnosis of phenylketonuria. (C) 2018 Published by Elsevier B.V.
dc.language.isoEnglish
dc.relation.ispartofINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
dc.subjectAptamer
dc.subjectGenetic disorder
dc.subjectBiomarker
dc.subjectPhenylketonuria
dc.subjectGold nanostructure
dc.titleAptamer-based assay for monitoring genetic disorder phenylketonuria (PKU)
dc.typeArticle
dc.citation.volume116
dc.citation.spage735
dc.citation.epage743
dc.citation.indexWeb of science
dc.identifier.DOIhttps://doi.org/10.1016/j.ijbiomac.2018.05.028


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