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dc.contributor.authorMahmoudpour, A
dc.contributor.authorRahimi, S
dc.contributor.authorSina, M
dc.contributor.authorSoroush, MH
dc.contributor.authorShahi, S
dc.contributor.authorShahisa, S
dc.contributor.authorAsl-Aminabadi, N
dc.date.accessioned2018-08-26T06:33:30Z
dc.date.available2018-08-26T06:33:30Z
dc.date.issued2007
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/43748
dc.description.abstractThis study was designed to survey the incidence of Enterococcus faecalis infection in symptomatic and asymptomatic root canals of necrotic teeth using PCR and to isolate the bacterium for further screening. Sixty patients categorized according to their clinical symptoms were used for sampling by insertion of paper points into the root canals and absorbing all the fluids present within them. The samples were incubated in 1.0 ml 2xYT (containing 16 g bacto tryptone, 10 g yeast extract and 5.0 g NaCl per liter) for 24 h at 37 degrees C without aeration prior to multiplex PCR analysis. To assist the isolation of E. faecalis, sub-samples were further grown in the same medium supplemented with 6.5% NaCl and back-inoculated into bile esculin. Using multiple cultivation-dependent and PCR analyses, 6 cases (10%) of E. faecalis were identified. Four isolates were obtained from asymptomatic cases of chronic apical periodontitis, and the other two were associated with phoenix abscess and acute apical abscess, respectively. No E. faecalis infection was found in 5 patients with acute apical periodontitis or in 9 with chronic suppurative periodontitis. Our results indicate that there is no significant difference in the incidence of E. faecalis between symptomatic and asymptomatic necrotic dental root canals (P > 0.05).
dc.language.isoEnglish
dc.relation.ispartofJournal of oral science
dc.subjectAdolescent
dc.subjectAdult
dc.subjectBacterial Typing Techniques
dc.subjectChild
dc.subjectDNA, Bacterial
dc.subjectDental Pulp Necrosis
dc.subjectElectrophoresis, Agar Gel
dc.subjectEnterococcus faecalis
dc.subjectFemale
dc.subjectGram-Positive Bacterial Infections
dc.subjectHumans
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectPeriapical Periodontitis
dc.subjectPolymerase Chain Reaction
dc.titleIsolation and identification of Enterococcus faecalis from necrotic root canals using multiplex PCR.
dc.typearticle
dc.citation.volume49
dc.citation.issue3
dc.citation.spage221
dc.citation.epage7
dc.citation.indexPubmed


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