dc.contributor.author | Ghanbarzadeh, S | |
dc.contributor.author | Arami, S | |
dc.contributor.author | Pourmoazzen, Z | |
dc.contributor.author | Khorrami, A | |
dc.date.accessioned | 2018-08-26T06:06:16Z | |
dc.date.available | 2018-08-26T06:06:16Z | |
dc.date.issued | 2014 | |
dc.identifier.uri | http://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/41967 | |
dc.description.abstract | pH-responsive polymers produce liposomes with pH-sensitive property which can release their encapsulated drug under mild acidic conditions found inside the cellular endosomes, inflammatory tissues and cancerous cells. The aim of this study was preparing pH-sensitive and plasma stable liposomes in order to enhance the selectivity and antiproliferative effect of Rapamycin. In the present study we used PEG-poly (monomethylitaconate)-CholC6 (PEG-PMMI-CholC6) copolymer and Oleic acid (OA) to induce pH-sensitive property in Rapamycin liposomes. pH-sensitive liposomal formulations bearing copolymer PEG-PMMI-CholC6 and OA were characterized in regard to physicochemical stability, pH-responsiveness and stability in human plasma. The ability of pH-sensitive liposomes in enhancing the cytotoxicity of Rapamycin was evaluated in vitro by using colon cancer cell line (HT-29) and compared with its cytotoxicity on human umbilical vein endothelial cell (HUVEC) line. Both formulations were found to release their contents under mild acidic conditions rapidly. However, unlike OA-based liposomes, the PEG-PMMI-CholC6 bearing liposomes preserved their pH-sensitivity in plasma. Both types of pH-sensitive Rapamycin-loaded liposomes exhibited high physicochemical stability and could deliver antiproliferative agent into HT-29 cells much more efficiently in comparison with conventional liposomes. Conversely, the antiproliferative effect of pH-sensitive liposomes on HUVEC cell line was less than conventional liposomes. This study showed that both OA and PEG-PMMI-CholC6-based vesicles could submit pH-sensitive property, however, only PEG-PMMI-CholC6-based liposomes could preserve pH-sensitive property after incubation in plasma. As a result pH-sensitive PEG-PMMI-CholC6-based liposomal formulation can improve the selectivity, stability and antiproliferative effect of Rapamycin. | |
dc.language.iso | English | |
dc.relation.ispartof | Colloids and surfaces. B, Biointerfaces | |
dc.subject | 1,2-Dipalmitoylphosphatidylcholine | |
dc.subject | Cell Line, Tumor | |
dc.subject | Cell Proliferation | |
dc.subject | Cholesterol | |
dc.subject | Drug Stability | |
dc.subject | Human Umbilical Vein Endothelial Cells | |
dc.subject | Humans | |
dc.subject | Hydrogen-Ion Concentration | |
dc.subject | Liposomes | |
dc.subject | Oleic Acid | |
dc.subject | Polyethylene Glycols | |
dc.subject | Polyvinyls | |
dc.subject | Sirolimus | |
dc.subject | Succinates | |
dc.title | Improvement of the antiproliferative effect of rapamycin on tumor cell lines by poly (monomethylitaconate)-based pH-sensitive, plasma stable liposomes. | |
dc.type | article | |
dc.citation.volume | 115 | |
dc.citation.spage | 323 | |
dc.citation.epage | 30 | |
dc.citation.index | Pubmed | |
dc.identifier.DOI | https://doi.org/10.1016/j.colsurfb.2013.12.024 | |