نمایش پرونده ساده آیتم

dc.contributor.authorBannazadeh Baghi, H
dc.contributor.authorNauwynck, HJ
dc.date.accessioned2018-08-26T05:41:17Z
dc.date.available2018-08-26T05:41:17Z
dc.date.issued2016
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/40482
dc.description.abstractThe respiratory mucosa is the common port of entry of equine herpesvirus type 1 (EHV-1) and several other alphaherpesviruses. An important prerequisite for successful host invasion of the virus is to cross the epithelial cell layer and the underlying basement membrane barrier. In the present study, an analysis was performed to see if an EHV-1 infection of nasal mucosa epithelial cells leads to damage of the underlying extracellular matrix proteins. Nasal mucosa explants were inoculated with EHV-1 and collected at 0, 24 and 48آ hours post-inoculation (hpi). Then, double immunofluorescence staining was performed to detect viral-antigen-positive cells on the one hand and integrin alpha 6, laminin, collagen IV and collagen VII on the other hand. The area of these extracellular matrix proteins was measured in regions of interest (ROIs) at a magnification of 200X by means of the software imaging system ImageJ. ROIs were defined beneath uninfected and infected regions. In uninfected regions, 22-28آ % of the ROI was stained for integrin alpha 6, 18-37آ % for laminin, 14-38آ % for collagen IV and 18-26آ % for collagen VII. In infected regions, the percentage positive for integrin alpha 6 was significantly decreased to 0.1-9آ % and 0.1-6آ % after 24 and 48آ hours of inoculation, respectively. Infection did not alter the percentages for laminin and collagen IV. For collagen VII, an increase in the percentage (from 18-26آ % to 28-39آ %) could be observed underneath EHV-1-infected plaques at 48آ hours of inoculation. In conclusion, the results revealed a substantial impact of EHV-1 infection on integrin alpha 6 and collagen VII, two important components of the extracellular matrix, which are associated with the basement membrane and may facilitate virus penetration via hijacked leukocytes to underlying tissues.
dc.language.isoEnglish
dc.relation.ispartofArchives of virology
dc.subjectAnimals
dc.subjectBasement Membrane
dc.subjectEpithelial Cells
dc.subjectHerpesviridae Infections
dc.subjectHerpesvirus 1, Equid
dc.subjectHorse Diseases
dc.subjectHorses
dc.subjectIntegrin alpha6
dc.subjectNasal Mucosa
dc.titleEffect of equine herpesvirus type 1 (EHV-1) infection of nasal mucosa epithelial cells on integrin alpha 6 and on different components of the basement membrane.
dc.typearticle
dc.citation.volume161
dc.citation.issue1
dc.citation.spage103
dc.citation.epage10
dc.citation.indexPubmed
dc.identifier.DOIhttps://doi.org/10.1007/s00705-015-2643-4


فایلهای درون آیتم

فایلهاسایزفرمتنمایش

هیچ فایل مرتبطی وجود ندارد

این آیتم در مجموعه های زیر مشاهده می شود

نمایش پرونده ساده آیتم