نمایش پرونده ساده آیتم

dc.contributor.authorSeidi, K
dc.contributor.authorJahanban-Esfahlan, R
dc.contributor.authorAbasi, M
dc.contributor.authorAbbasi, MM
dc.date.accessioned2018-08-26T05:38:44Z
dc.date.available2018-08-26T05:38:44Z
dc.date.issued2016
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/40093
dc.description.abstractPunica granatum (PG) has been demonstrated to possess antitumor effects on various types of cancer cells. In this study, we determined antiproliferative properties of a seed extract of PG (PSE) from Iran in different human cancer cells.A methanolic extract of pomegranate seeds was prepared. Total phenolic content (TPC) and total flavonoid content (TFC) were assessed by colorimetric assays. Antioxidant activity was determined with reference to DPPH radical scavenging activity. The cytotoxicity of different doses of PSE (0, 5, 20, 100, 250, 500, 1000 ?g/ml) was evaluated by MTT assays with A549 (lung non small cell carcinoma), MCF-7 (breast adenocarcinoma), SKOV3 (ovarian cancer cells), and PC-3 (prostate adenocarcinoma) cells.Significant (P<0.01) or very significant (P<0.0001) differences were observed in comparison to negative controls at all tested doses (5-1000 ?g/ml). In all studied cancer cells, PSE reduced the cell viability to values below 23%, even at the lowest doses. In all cases, IC50 was determined at doses below 5 ?g/ml. In this regard, SKOV3 ovarian cancer cells were the most responsive to antiproliferative effects of PSE with a maximum mean growth inhibition of 86.8% vs. 82.8%, 81.4% and 80.0% in MCF-7, PC-3 and A549 cells, respectively.Low doses of PSE exert potent antiproliferative effects on different human cancer cells SKOV3 ovarian cancer cells as most and A549 cells ar least responsive regarding cytotoxic effects. However, the mechanisms of action need to be addressed.
dc.language.isoEnglish
dc.relation.ispartofAsian Pacific journal of cancer prevention : APJCP
dc.subjectA549 Cells
dc.subjectAntineoplastic Agents
dc.subjectAntioxidants
dc.subjectApoptosis
dc.subjectCell Line, Tumor
dc.subjectCell Proliferation
dc.subjectCell Survival
dc.subjectFlavonoids
dc.subjectHumans
dc.subjectMCF-7 Cells
dc.subjectNeoplasms
dc.subjectPhenols
dc.subjectPlant Extracts
dc.subjectPunicaceae
dc.subjectSeeds
dc.titleAnti Tumoral Properties of Punica granatum (Pomegranate) Seed Extract in Different Human Cancer Cells.
dc.typearticle
dc.citation.volume17
dc.citation.issue3
dc.citation.spage1119
dc.citation.epage22
dc.citation.indexPubmed


فایلهای درون آیتم

فایلهاسایزفرمتنمایش

هیچ فایل مرتبطی وجود ندارد

این آیتم در مجموعه های زیر مشاهده می شود

نمایش پرونده ساده آیتم