Evaluating -238 G>A Polymorphism Association in TNF-? Gene with Metabolic Parameters and Nutritional Intakes Among the Iranian NAFLD Patients.

Abstract

Different studies have shown that -238 G>A polymorphism in promoter region of tumor necrosis factor alpha (TNF-?) gene is associated with increased risk of non-alcoholic fatty liver disease (NAFLD). The current study investigates the association between metabolic parameters and nutritional intakes with -238 G>A of TNF-? promoter gene polymorphism among the Iranian patients with NAFLD. In this study, 75 patients with NAFLD and 76 individuals as control were enrolled. Dietary intakes were assessed using a semi-quantitative food-frequency questionnaire. Body mass index and waist to hip ratio were calculated. Biochemical assays were measured after 12آ h fasting. -238 G>A Polymorphism of TNF-? gene was determined by using sequencing method. We observed no significant difference in frequency of different genotypes of this polymorphism between NAFLD and control groups (Pآ >آ 0.05). Among biochemical parameters, TAC showed significant decrease in NAFLD patients with GG genotype when compared to controls (Pآ =آ 0.001). The comparison of macro and micronutrient intakes between groups according to genotypes showed no statistically significant difference (Pآ >آ 0.05). Although the data were not statistically significant, further studies with larger sample size are needed to determine the effect of dietary compounds in NAFLD.