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dc.contributor.authorGholizadeh-Ghaleh Aziz, S
dc.contributor.authorPashaei-Asl, F
dc.contributor.authorFardyazar, Z
dc.contributor.authorPashaiasl, M
dc.date.accessioned2018-08-26T05:37:13Z
dc.date.available2018-08-26T05:37:13Z
dc.date.issued2016
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/39805
dc.description.abstractHuman stem cells and progenitor cells can be used to treat cancer and replace dysfunctional cells within a tissue or organ. The objective of this study was to identify the appropriate cells type in regenerative medicine and targeted therapy. As an alternative to embryonic and bone marrow stem cells, we examined human amniotic fluid stem cells (hAFSCs), one of the potential source of multipotent stem cells isolated from both cell pellet (using single-stage method), and supernatant of human amniotic fluid. Source of isolation and unique property of the cells emphasize that these cells are one of the promising new tools in therapeutic field. Double sources for isolation and availability of the left over samples in diagnostic laboratory at the same time have less legal and ethical concerns compared with embryonic stem cell studies. Cells were isolated, cultured for 18th passage for 6 months and characterized using qPCR and flow cytometry. Cells showed good proliferative ability in culture condition. The cells successfully differentiated into the adipogenic and osteogenic lineages. Based on these findings, amniotic fluid can be considered as an appropriate and convenient source of human amniotic fluid stem cells. These cells provide potential tools for therapeutic applications in the field of regenerative medicine. To get a better understanding of crosstalk between Oct4/NANOG with osteogenesis and adipogenesis, we used network analysis based on Common Targets algorithm and Common Regulators algorithm as well as subnetwork discovery based on gene set enrichment. Network analysis highlighted the possible role of MIR 302A and MIR let-7g. We demonstrated the high expression of MIR 302A and low expression of MIR let7g in hAFSCs by qPCR.
dc.language.isoEnglish
dc.relation.ispartofPloS one
dc.subjectAdipocytes
dc.subjectAmniotic Fluid
dc.subjectCell Differentiation
dc.subjectCell Proliferation
dc.subjectCryopreservation
dc.subjectGene Expression Profiling
dc.subjectGene Expression Regulation
dc.subjectGene Regulatory Networks
dc.subjectHumans
dc.subjectMicroRNAs
dc.subjectMultipotent Stem Cells
dc.subjectNanog Homeobox Protein
dc.subjectOctamer Transcription Factor-3
dc.subjectOsteoblasts
dc.subjectPrimary Cell Culture
dc.titleIsolation, Characterization, Cryopreservation of Human Amniotic Stem Cells and Differentiation to Osteogenic and Adipogenic Cells.
dc.typearticle
dc.citation.volume11
dc.citation.issue7
dc.citation.spagee0158281
dc.citation.indexPubmed
dc.identifier.DOIhttps://doi.org/10.1371/journal.pone.0158281


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