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dc.contributor.authorMoosavi, MA
dc.contributor.authorSharifi, M
dc.contributor.authorGhafary, SM
dc.contributor.authorMohammadalipour, Z
dc.contributor.authorKhataee, A
dc.contributor.authorRahmati, M
dc.contributor.authorHajjaran, S
dc.contributor.author?os, MJ
dc.contributor.authorKlonisch, T
dc.contributor.authorGhavami, S
dc.date.accessioned2018-08-26T05:36:39Z
dc.date.available2018-08-26T05:36:39Z
dc.date.issued2016
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/39613
dc.description.abstractIn this study, we used nitrogen-doped titanium dioxide (N-TiO2) NPs in conjugation with visible light, and show that both reactive oxygen species (ROS) and autophagy are induced by this novel NP-based photodynamic therapy (PDT) system. While well-dispersed N-TiO2 NPs (?100??g/ml) were inert, their photo-activation with visible light led to ROS-mediated autophagy in leukemia K562 cells and normal peripheral lymphocytes, and this increased in parallel with increasing NP concentrations and light doses. At a constant light energy (12?J/cm2), increasing N-TiO2 NP concentrations increased ROS levels to trigger autophagy-dependent megakaryocytic terminal differentiation in K562 cells. By contrast, an ROS challenge induced by high N-TiO2 NP concentrations led to autophagy-associated apoptotic cell death. Using chemical autophagy inhibitors (3-methyladenine and Bafilomycin A1), we confirmed that autophagy is required for both terminal differentiation and apoptosis induced by photo-activated N-TiO2. Pre-incubation of leukemic cells with ROS scavengers muted the effect of N-TiO2 NP-based PDT on cell fate, highlighting the upstream role of ROS in our system. In summary, PDT using N-TiO2 NPs provides an effective method of priming autophagy by ROS induction. The capability of photo-activated N-TiO2 NPs in obtaining desirable cellular outcomes represents a novel therapeutic strategy of cancer cells.
dc.language.isoEnglish
dc.relation.ispartofScientific reports
dc.subjectAutophagy
dc.subjectCell Differentiation
dc.subjectHumans
dc.subjectK562 Cells
dc.subjectLeukemia
dc.subjectNanoparticles
dc.subjectNitrogen
dc.subjectPhotochemotherapy
dc.subjectReactive Oxygen Species
dc.subjectTitanium
dc.titlePhotodynamic N-TiO2 Nanoparticle Treatment Induces Controlled ROS-mediated Autophagy and Terminal Differentiation of Leukemia Cells.
dc.typearticle
dc.citation.volume6
dc.citation.spage34413
dc.citation.indexPubmed
dc.identifier.DOIhttps://doi.org/10.1038/srep34413


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