Association of CD46 IVS1-1724 C>G Single Nucleotide Polymorphism in Iranian Women with Unexplained Recurrent Spontaneous Abortion (URSA).

Abstract

There are several known and unknown factors for unexplained recurrent spontaneous abortion (URSA). Among them, complement regulatory protein CD46 plays a pivotal role in preventing uncontrolled activation of complement and successful continuation of pregnancy. We aimed in this study to investigate the possible association of CD46 IVS1-1724 C>G polymorphism with RSA in Iranian women. 141 women with RSA and 153 women with normal pregnancy were enrolled in this study. RSA was confirmed as the history of having at least three consecutive abortions without any known immunologic, pathologic and genetic reason. Genomic DNA was extracted and RFLP-PCR was done using a specific primer pair and HindIII restriction enzyme. Statistical analysis was done for determining the genotype and allele frequency, and also for odds ratio (OR). Statistical analysis showed no significant difference in genotype frequency between two RSA and normal groups. However G allele was significantly more frequent in fertile women and represented as a protective allele (p=0.04, OR=0.8, CI 95%). In contrary to similar studies in other two ethnic populations, our study showed no genotype differences in CD46 IVS1-1724 C>G Single nucleotide polymorphis (SNP) between RSA and fertile women. On the other hand, G allele was revealed as a protective allele for RSA. CD46 polymorphisms may predict the outcome of pregnancy; however, more studies in different ethnic groups are required.