نمایش پرونده ساده آیتم

dc.contributor.authorTohidkia, MR
dc.contributor.authorSepehri, M
dc.contributor.authorKhajeh, S
dc.contributor.authorBarar, J
dc.contributor.authorOmidi, Y
dc.date.accessioned2018-08-26T05:01:27Z
dc.date.available2018-08-26T05:01:27Z
dc.date.issued2017
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/39033
dc.description.abstractPhage display technology (PDT) is a powerful tool for the isolation of recombinant antibody (Ab) fragments. Using PDT, target molecule-specific phage-Ab clones are enriched through the "biopanning" process. The individual specific binders are screened by the monoclonal scFv enzyme-linked immunosorbent assay (ELISA) that may associate with inevitable false-negative results. Thus, in this study, three strategies were investigated for optimization of the scFvs screening using Tomlinson I and J libraries, including (1) optimizing the expression of functional scFvs, (2) improving the sensitivity of ELISA, and (3) preparing different samples containing scFvs. The expression of all scFv Abs was significantly enhanced when scFv clones were cultivated in the terrific broth (TB) medium at the optimum temperature of 30 °C. The protein A-conjugated with horseradish peroxidase (HRP) was found to be a well-suited reagent for the detection of Ag-bound scFvs in comparison with either anti-c-myc Ab or the mixing procedure. Based on our findings, it seems there is no universal media supplement for an improved expression of all scFvs derived from both Tomlinson I and J libraries. We thus propose that expression of scFv fragments in a microplate scale is largely dependent on a variety of parameters, in particular the scFv clones and relevant sequences.
dc.language.isoEnglish
dc.relation.ispartofSLAS discovery : advancing life sciences R & D
dc.titleImproved Soluble ScFv ELISA Screening Approach for Antibody Discovery Using Phage Display Technology.
dc.typearticle
dc.citation.volume22
dc.citation.issue8
dc.citation.spage1026
dc.citation.epage1034
dc.citation.indexPubmed
dc.identifier.DOIhttps://doi.org/10.1177/2472555217701059


فایلهای درون آیتم

فایلهاسایزفرمتنمایش

هیچ فایل مرتبطی وجود ندارد

این آیتم در مجموعه های زیر مشاهده می شود

نمایش پرونده ساده آیتم