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dc.contributor.authorPhan, JA
dc.contributor.authorStokholm, K
dc.contributor.authorZareba-Paslawska, J
dc.contributor.authorJakobsen, S
dc.contributor.authorVang, K
dc.contributor.authorGjedde, A
dc.contributor.authorLandau, AM
dc.contributor.authorRomero-Ramos, M
dc.date.accessioned2018-08-26T04:57:59Z
dc.date.available2018-08-26T04:57:59Z
dc.date.issued2017
dc.identifier.urihttp://dspace.tbzmed.ac.ir:8080/xmlui/handle/123456789/38694
dc.description.abstractEvidence suggests that synapses are affected first in Parkinson's disease (PD). Here, we tested the claim that pathological accumulation of ?-synuclein, and subsequent synaptic disruption, occur in absence of dopaminergic neuron loss in PD. We determined early synaptic changes in rats that overexpress human ?-synuclein by local injection of viral-vectors in midbrain. We aimed to achieve ?-synuclein levels sufficient to induce terminal pathology without significant loss of nigral neurons. We tested synaptic disruption in vivo by analyzing motor defects and binding of a positron emission tomography (PET) radioligand to the vesicular monoamine transporter 2, (VMAT2), [11C]dihydrotetrabenazine (DTBZ). Animals overexpressing ?-synuclein had progressive motor impairment and, 12 weeks post-surgery, showed asymmetric in vivo striatal DTBZ binding. The PET images matched ligand binding in post-mortem tissue, and histological markers of dopaminergic integrity. Histology confirmed the absence of nigral cell death with concomitant significant loss of striatal terminals. Progressive aggregation of proteinase-K resistant and Ser129-phosphorylated ?-synuclein was observed in dopaminergic terminals, in dystrophic swellings that resembled axonal spheroids and contained mitochondria and vesicular proteins. In conclusion, pathological ?-synuclein in nigro-striatal axonal terminals leads to early axonal pathology, synaptic disruption, dysfunction of dopaminergic neurotransmission, motor impairment, and measurable change of VMAT2 in the absence of cell loss.
dc.language.isoEnglish
dc.relation.ispartofScientific reports
dc.titleEarly synaptic dysfunction induced by ?-synuclein in a rat model of Parkinson's disease.
dc.typearticle
dc.citation.volume7
dc.citation.issue1
dc.citation.spage6363
dc.citation.indexPubmed
dc.identifier.DOIhttps://doi.org/10.1038/s41598-017-06724-9


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