New Developments in Pichia pastoris Expression System, Review and Update.

Abstract

Although Pichia pastoris is an outstanding host among conventional expression systems for production of recombinant proteins, a new interest have been emerged to this system due to the inherent advantages and new developments in this expression host. The potential for secretory and soluble expression of heterologous glycoproteins in P. pastoris proposed this system as a candidate for the production of complex eukaryotic proteins.Several new developments have been occurred in different areas related to P. pastoris expression system including hosts, vectors, glycosylation pattern and fermentation technology. Strain engineering using Crispr/Cas9 technology to produce human-like glycoproteins and protease deficient strains are two new areas of development with high importance.This review dedicated to discuss on the most important characteristics of P. pastoris with emphasizes on new developments, especially in the field of glycoengineering, efficient expression vectors and promoters.New developments occurred in the P. pastoris expression system converted this system to a versatile host for production of complex proteins. These progresses paved the way for several proteins to enter to the clinical trials or industrial processes from this valuable expression host.